Table I.

List of flower cutin monomers identified after BF3 depolymerization with their respective concentrations in dcr-1 mutants and wild-type plants

Data presented here are in μg mg−1 dry weight and represent averages of four replicates. Differences with P < 0.05 were regarded as significant.

Identified Cutin Monomersdcr-1Wild Type (Nossen)Change versus the Wild Type
MeansdMeansd
Aromatics
    trans-Coumaric acid0.03770.01180.10530.0148Down
    trans-Ferulic acid0.10020.00790.10750.0087
    cis-Sinapic acid0.03170.00590.01830.0028
    trans-Sinapic acid0.26000.00480.30260.0077Down
    Subtotal0.42970.03040.53360.0339
Saturated fatty acids
    C24-FA (tetracosanoic acid)0.06700.01470.09220.0024
Long-chain alcohols
    C20-1-ol (docosanol)0.20590.00270.18190.0185
Dicarboxylic fatty acids
    C16:2-DA [hexadecadiene-(1,16)-dioic acid]0.07180.00130.08370.0071
    C16:1-DA [hexadecene-(1,16)-dioic acid]0.07570.02620.06590.0050
    C16-DA [hexadecane-(1,16)-dioic acid]2.46040.07630.77690.0274Up
    C18:2-DA [octadecadiene-(1,18)-dioic acid]0.85230.03241.22070.1288
    C18:1-DA [octadecene-(1,18)-dioic acid]0.99940.04070.99810.0459
    C18-DA [octadecane-(1,18)-dioic acid]0.28700.00510.24540.0375
    Subtotal4.74660.18193.39060.2518
Mid-chain hydroxylated fatty acids
    C16-9/10-HDA [9/10-hydroxy-hexadecane-(1,16)-dioic acid]0.24530.00800.78400.1134Down
    C16-9/10,16-DHFA (9/10,16-dihydroxy-hexadecanoic acid)0.05420.04099.73991.2405Down
    Subtotal0.29940.048810.52401.3538Down
Terminal-hydroxylated fatty acids
    C16:1-ω-HFA (16-hydroxy-hexadecenoic acid)0.16830.05270.16370.0216
    C16-ω-HFA (16-hydroxy-hexadecanoic acid)0.83620.02580.54420.0336Up
    C18:3-ω-HFA (18-hydroxy-octadecatrienoic acid)0.30590.07140.56710.1445
    C18:2-ω-HFA (18-hydroxy-octadecadienoic acid)0.12070.00330.39540.0446Down
    C18:1-ω-HFA (18-hydroxy-octadecenoic acid)0.04850.00160.15490.0224Down
    Subtotal1.47960.15471.82540.2666
2-Hydroxylated fatty acids
    C16-2-HFA (2-hydroxy-hexadecanoic acid)0.04520.00060.03960.0008Up
    C22:1-2-HFA (2-hydroxy-docosenoic acid)0.04360.01470.11620.0066Down
    C22-2-HFA (2-hydroxy-docosanoic acid)0.17250.00620.15140.0021Up
    C24:1-2-HFA (2-hydroxy-tetracosenoic acid)0.26370.01310.25630.0042
    C24-2-HFA (2-hydroxy-tetracosanoic acid)0.39340.00400.28250.0862
    C26:1-2-HFA (2-hydroxy-hexacosenoic acid)0.06850.00310.06640.0035
    C26-2-HFA (2-hydroxy-hexacosanoic acid)0.14730.00380.12960.0027Up
    Subtotal1.13430.04551.04210.1062
Total8.36240.478717.58982.0332Down