Table II.

Incomplete dephosphorylation of insoluble phosphoglucans using varying levels of AtSEX4

The B-type allomorph of MDcryst, prephosphorylated with StGWD and [β-33P]ATP for 60 min, was incubated with either 25 or 100 ng of AtSEX4. At intervals, the levels of [33P]orthophosphate were determined. [33P]Orthophosphate released is given as percentage of the total 33P content of the prephosphorylated MD. Mean values n = 3; ±sd.

Time33P-Orthophosphate Released
25 ng SEX4100 ng SEX4
min%
530 ± 333 ± 2
1036 ± 236 ± 4
3038 ± 341 ± 4
6040 ± 346 ± 3