Table VI.

Orthophosphate release from ATP by AtSEX4 and Antarctic phosphatase

Both phosphatases were balanced to equal enzyme activity using p-nitrophenyl phosphate as substrate. [33P]Orthophosphate, as determined by TLC and phosphor imaging, is given as percentage of the total 33P content of the reaction mixture (n = 3; ±sd). n.d., Below the limit of detection.

Time33P-Orthophosphate Released
SEX4Antarctic Phosphatase
min%
1n.d.15 ± 2
2n.d.29 ± 0.4
51 ± 0.265 ± 4.8
103 ± 0.186 ± 2.5
306 ± 0.488 ± 1.2
606 ± 0.896 ± 0.2