Table VII.

Simultaneous action of AtSEX4 and StGWD on prelabeled insoluble (phospho)glucans

The B-type allomorph of MDcryst was prephosphorylated (and prelabeled) for 5 or 60 min using StGWD and [β-33P]ATP. Subsequently, the insoluble MD were incubated for 60 min with StGWD, AtSEX4, or StGWD plus AtSEX4. At intervals, aliquots were centrifuged and the supernatants were collected. The content of [33P]orthophosphate, 33P-phosphoglucans, and of the total glucosyl residues was determined. n.d., Below the limit of detection. Mean values are given (n = 3; ±sd).

Incubation TimePreincubation
5 min60 min5 min60 min5 min60 min
33P-Orthophosphate Released [pmol]33P in Soluble Phosphoglucans [pmol]Glc Residues Released [nmol]
min
StGWD0n.d.n.d.n.d.0.8n.d.0.5
10n.d.n.d.0.0439.2n.d.6.1
20n.d.n.d.0.08118.0n.d.2.2
30n.d.n.d.0.09026.40.2244.4
40n.d.n.d.0.10527.245.544.4
50n.d.n.d.0.18930.446.650.0
60n.d.n.d.0.23431.255.555.0
AtSEX40n.d.n.d.n.d.n.d.n.d.n.d.
100.088.4n.d.n.d.n.d.n.d.
200.1613.6n.d.n.d.n.d.n.d.
300.2017.6n.d.n.d.n.d.n.d.
400.2218.4n.d.n.d.n.d.n.d.
500.2219.2n.dn.dn.dn.d
600.2319.2n.d.n.d.n.d.n.d.
StGWD + AtSEX40n.d.n.d.n.d.n.d.n.d.n.d
100.5521.6n.d.n.d.n.d.n.d.
200.7529.6n.d.n.d.n.d.n.d.
300.8032.4n.d.n.d.n.d.n.d.
400.8434.0n.d.n.d.0.130.33
500.8635.2n.d.n.d.0.270.50
600.8635.2n.d.n.d.0.270.55