Table I.

Purification of CS-LIKE expressed in E. coli

The gene encoding CS-LIKE was cloned in the pDEST17 vector using the Gateway technology. The protein was expressed in E. coli and purified using the Ni-NTA purification system as described in “Materials and Methods.” DES and OASTL activities were measured as described.

Purification StepProteinSpecific ActivityTotal ActivityPurification FactorYield
DESOASTLDESOASTLDESOASTLDESOASTL
mgunits mg−1units%
Crude extract1.2910.08.813.011.3
Ni-NTA chromatography0.1644.51.27.10.24.50.154.61.7