Table III.

Enzyme activity and thiol content

DES enzyme activity and total levels of Cys and glutathione were measured in the leaves of Col-0 and No-0 wild-type and des1 mutant plants grown for 5 weeks in soil under control conditions and in whole 2-week-old seedlings grown on MS solid medium. Values are means ± sd of four independent experiments made from different plants and seedlings grown in different pots and plates, respectively. ANOVA was performed using the software OriginPro 7.5. ** P < 0.05

Plant LineDES ActivityTotal CysTotal Glutathione
nmol min−1 mg−1nmol g−1 fresh wt
Leaves from 5-week-old plants grown in soil
    Col-012.4 ± 0.829.8 ± 2.3302.8 ± 21.8
    des1-110.1 ± 0.7**36.1 ± 4.6**345.2 ± 12.3**
    No-015.5 ± 0.929.0 ± 3.4322.2 ± 11.3
    des1-213.1 ± 0.8**36.2 ± 7.3**390.0 ± 16.2**
Whole 2-week-old seedlings grown on MS medium
    Col-06.9 ± 0.0547.7 ± 4.1730.7 ± 51.2
    des1-15.9 ± 0.1**58.0 ± 3.8**867.2 ± 10.2**
    No-07.15 ± 0.2850.3 ± 2.4606.6 ± 14.6
    des1-26.2 ± 0.4**62.9 ± 2.8**693.3 ± 13.7**