Table I.

Relative fluorescence of dyes in the presence of carbohydrates

The fluorescence intensity of S4B and 7GFE increases in the presence of specific carbohydrates. Fluorescence of S4B, 7GFE, or Calcofluor incubated with carbohydrates (see “Materials and Methods” for sources) relative to their fluorescence in control solutions (water or PBS). X = excitation wavelength, M = emission wavelength. Each dye's relative fluorescence values are standardized to its own control value. Errors are sd from 12 wells for each mixture.

CarbohydrateS4B7GFECalcofluorCalcofluor
X = 535 nm, M = 595 nmX = 485 nm, M = 535 nmX = 360 nm, M = 465 nmX = 485 nm, M = 535 nm
PBS control1.00 ± 0.04481.00 ± 0.019
Water control1.00 ± 0.05821.00 ± 0.281
Cellulose64.4 ± 0.95639.0 ± 7.112.53 ± 0.22819.3 ± 2.84
Xyloglucan14.6 ± 2.9312.8 ± 4.353.75 ± 0.2281.47 ± 0.428
Curdlan3.28 ± 0.2003.03 ± 0.8961.88 ± 0.6220.965 ± 0.230
Glucomannan1.02 ± 0.5288.17 ± 0.5480.962 ± 0.5481.47 ± 0.0618
Lichenan1.26 ± 0.5048.93 ± 1.163.50 ± 0.61915.2 ± 2.51
Arabinan1.20 ± 0.08450.776 ± 0.1140.751 ± 0.03679.01 ± 0.473
Arabinogalactan1.13 ± 0.1021.05 ± 0.08990.909 ± 0.05241.86 ± 0.0875
Arabinoxylan0.951 ± 0.08020.918 ± 0.1060.841 ± 0.02783.19 ± 1.50
Cellobiose0.856 ± 0.1000.870 ± 0.09370.917 ± 0.07630.901 ± 0.113
Cellotriose0.963 ± 0.09100.836 ± 0.1071.12 ± 0.1051.04 ± 0.0965
Cellotetrose0.834 ± 0.08420.889 ± 0.1130.973 ± 0.06390.882 ± 0.152
Cellopentose1.15 ± 0.09831.17 ± 0.09050.812 ± 0.1010.888 ± 0.0929
Cellohexose0.940 ± 0.1040.914 ± 0.1051.12 ± 0.1580.915 ± 0.113
Galactomannan0.714 ± 0.1091.20 ± 0.1010.762 ± 0.066427.9 ± 1.59
Isoprimeverose1.27 ± 0.1070.835 ± 0.09381.03 ± 0.05741.34 ± 0.297
Mannan1.23 ± 0.09751.10 ± 0.08840.947 ± 0.04524.09 ± 0.259
Pectic galactan0.821 ± 0.09301.04 ± 0.1170.962 ± 0.0449117.6 ± 3.74
Rhamnogalacturonan1.17 ± 0.08841.01 ± 0.09980.873 ± 0.09660.873 ± 0.0966
Starch0.837 ± 0.09320.941 ± 0.08380.911 ± 0.06551.82 ± 0.211
Xylan1.08 ± 0.08101.05 ± 0.1031.47 ± 0.054510.5 ± 0.506