Table I. PCR genotyping of segregating impl2-1 and impl2-2 populations

χ2 tests were used to determine whether the ratio of heterozygous to wild-type individuals was significantly different from 2:1.

Insertion LineWild TypeHeterozygoteHomozygoteχ2 ValueP Value
impl2-1173000.17020.68
impl2-2234500.00750.93