Table I. Validation of enzyme parameters determined by parameter estimation

Values of Km and Ki are given in mm. The unit of maximum enzyme activity (Vmax) is μmol h−1 g−1 fresh weight. F6P, Fru-6-P; FrcK, fructokinase; G6P, Glc-6-P; GlcK, glucokinase; Inv, invertase.

ReactionParameterGenotypeParameter EstimationExperimental DataLiterature Values
ValueSpeciesReference
rSP→Suc (SPS)Vmax (5 am)Col-00.4 ± 0.23.7 ± 3.632.4ArabidopsisGibon et al. (2004a)
inv43.9 ± 6.85.5 ± 0.9
Vmax (1 pm)Col-027.1 ± 1.430.2 ± 5.1
inv421.9 ± 1.023.8 ± 4.0
Vmax (9 pm)Col-012.2 ± 1.39.6 ± 4.1
inv412.3 ± 1.38.8 ± 2.9
KmCol-00.53 ± 0.0820.7–0.8Spinacia oleraceaHarbron et al. (1981)
inv4
rSuc→Hex (Inv)Vmax (5 am)Col-068.0 ± 6.959.3 ± 18.460ArabidopsisKlotke et al. (2004)
inv411.0 ± 0.0017.9 ± 3.2
Vmax (1 pm)Col-0155.5 ± 12.0137.0 ± 29.0
inv412.9 ± 0.28.6 ± 4.930,000Tang et al. (1996)
Vmax (9 pm)Col-099.9 ± 7.5114.7 ± 20.6
inv417.0 ± 1.815.6 ± 4.3
KmCol-011.7 ± 1.215–12ArabidopsisTang et al. (1996)
inv4
Ki,GlcCol-00.01 ± 0.001
inv4
Ki,FruCol-00.78 ± 2.292.5Lolium temulentumKingston-Smith et al. (1999)
inv4
rFru→SP (FrcK)Vmax (5 am)Col-04.1 ± 0.54.61 ± 1.315.0 ± 0.5ArabidopsisMoore et al. (2003)
inv44.9 ± 1.65.0 ± 0.4
Vmax (1 pm)Col-03.6 ± 0.64.0 ± 0.8
inv44.4 ± 1.23.1 ± 0.8
Vmax (9 pm)Col-08.0 ± 1.39.2 ± 0.4
inv48.8 ± 2.67.8 ± 1.9
KmCol-00.8 ± 0.0751.5–30S. oleraceaClaeyssen and Rivoal (2007)
inv4
Ki,F6PCol-01.97 ± 0.31
inv4
rGlc→SP (GlcK)Vmax (5 am)Col-04.8 ± 0.94.8 ± 1.33.8 ± 0.2ArabidopsisMoore et al. (2003)
inv44.5 ± 1.54.1 ± 0.4
Vmax (1 pm)Col-06.2 ± 1.73.4 ± 1.6
inv45.2 ± 1.53.8 ± 0.1
Vmax (9 pm)Col-012.9 ± 2.810.1 ± 1.9
inv47.9 ± 2.48.6 ± 0.4
KmCol-00.1 ± 0.020.02–0.13S. oleraceaClaeyssen and Rivoal (2007)
inv4
Ki,G6PCol-00.14 ± 0.03No effectPisum sativumClaeyssen and Rivoal (2007)
inv4Weak effectZea mays