Table II. Diurnal dynamics of identified enzyme parameters represented in the model

Values of Km and Ki are given in mm. The unit of maximum enzyme activity (Vmax) is μmol h−1 g−1 fresh weight. Asterisks indicate significant differences from the wild type (* P < 0.01, ** P < 0.001).

GenotypeReactionParameterTime
9 am1 pm5 pm9 pm1 am5 am
Col-0rSP→SucVmax27.9 ± 1.827.1 ± 1.421.9 ± 2.212.2 ± 1.34.8 ± 0.30.4 ± 0.2
Km: 0.53 ± 0.082
rSuc→HexVmax68.3 ± 8.1155.5 ± 12.0141.6 ± 12.199.9 ± 7.570.2 ± 5.568.0 ± 6.9
Km: 11.7 ± 1.21
Ki,Glc: 0.01 ± 0.001
Ki,Fru: 0.78 ± 2.29
rFru→SPVmax3.5 ± 0.53.6 ± 0.67.9 ± 1.38.0 ± 1.38.1 ± 1.44.1 ± 0.5
Km: 0.8 ± 0.075
Ki: 1.97 ± 0.31
rGlc→SPVmax6.1 ± 1.06.2 ± 1.712.4 ± 2.612.9 ± 2.810.27 ± 2.34.83 ± 0.9
Km: 0.1 ± 0.02
Ki: 0.14 ± 0.03
inv4rSP→SucVmax23.1 ± 1.0*21.9 ± 1.0*17.0 ± 1.2*12.3 ± 1.33.0 ± 1.1*3.9 ± 6.8
Km: 0.27 ± 0.02
rSuc→HexVmax12.9 ± 0.2**12.9 ± 0.2**15.0 ± 0.001**17.0 ± 1.8**11.8 ± 1.3**11.0 ± 0.001**
Km: 8.12 ± 2.74
Ki,Glc: 0.07 ± 0.06
Ki,Fru: 1.9 ± 4
rFru→SPVmax5.8 ± 1.8*4.4 ± 1.2*9.5 ± 2.88.8 ± 2.67.1 ± 2.5*4.9 ± 1.6
Km: 1.17 ± 0.27
Ki: 2.16 ± 0.47
rGlc→SPVmax6.0 ± 1.85.2 ± 1.5*9.5 ± 2.8*7.9 ± 2.4*6.7 ± 2.3*4.5 ± 1.5
Km: 0.08 ± 0.02
Ki: 0.147 ± 0.016