Table IV. Effects of additives on HvUXE1 reaction rates

The assay was conducted for 20 min at 25°C in 50 mm sodium phosphate buffer, pH 7.0. The data are means of three replicates ± sd. The activity was 470 pmol min−1 mg−1 protein in the reaction mixture containing only sodium phosphate buffer, 0.5 mm UDP-Xylp, and recombinant HvUXE1 (0.14 μg).

AdditivePercentage
None100 ± 4
DTT (2 mm)150 ± 8
EDTA (2 mm)95 ± 2
MgCl2 (1 mm)101 ± 2
NAD+ (1 mm)105 ± 2
NADH (1 mm)90 ± 3
UMP (2 mm)93 ± 1
UDP (2 mm)15 ± 1
UTP (2 mm)54 ± 3