Roots for mitochondrial mMDH, FL, SCoAL, ACO, and CS plants were incubated for 5 h. Two representative lines of each transgenic set were characterized. Values are presented as means ± se of six individual plants per line. Values marked with different letters differ significantly from each other (P < 0.05). Negligible enrichment is indicated with “nd.”
| Genotype | Citrate | Fumarate | Isocitrate (×10−4) | Malate | 2-Oxoglutarate |
| μmol label accumulated expressed as hexose equivalents | |||||
| Wild type | 31.7 ± 10.3 | 0.7 ± 0.1 | 2.7 ± 0.9 | 4.7 ± 0.9 | 2.7 ± 1.2 |
| mMDH7 | 15.6 ± 4.7 | 0.3 ± 0.2 | 1.1 ± 0.3a | 3.3 ± 1.8 | 5.4 ± 1.9 |
| mMDH8 | 69.0 ± 21.4 | 1.1 ± 0.6 | 2.9 ± 1.0 | 8.9 ± 1.6a | 17.6 ± 3.4a |
| mMDH21 | 7.3 ± 2.7a | 0.2 ± 0.1a | 3.3 ± 1.3 | 2.6 ± 0.8 | 1.0 ± 0.3 |
| FL11 | 7.5 ± 2.0a | 0.3 ± 0.2 | 7.5 ± 2.1b | 0.9 ± 0.3b | 1.8 ± 1 |
| FL41 | 5.2 ± 2.6a | 0.5 ± 0.1 | 4.4 ± 2.3 | 0.5 ± 0.2b | 2.8 ± 1.1 |
| FL63 | 26.2 ± 12.6 | 0.2 ± 0.1a | 18.1 ± 9.3 | 4 ± 1.3 | 18.4 ± 2.1a |
| SCoAL18 | 38.8 ± 4.8 | 0.8 ± 0.3 | nd | 2.3 ± 0.3c | 7.7 ± 1.0b |
| SCoAL25 | 33.7 ± 3.7 | 0.4 ± 0.1 | nd | 3.8 ± 1.1 | 3.5 ± 1.1 |
| SCoAL47 | 26.5 ± 5.7 | 0.7 ± 0.3 | nd | 5.5 ± 2.5 | 3.5 ± 2.0 |
| ACO19 | 20.6 ± 5.3 | 0.5 ± 0.1 | nd | 4.3 ± 1.0 | 2.4 ± 0.7 |
| ACO26 | 25 ± 8.3 | 0.8 ± 0.3 | nd | 6.6 ± 1.5 | 1.3 ± 0.2 |
| ACO38 | 14.6 ± 3.2b | 0.7 ± 0.4 | nd | 5.8 ± 2.0 | 2.4 ± 1.1 |
| CS22 | 32.9 ± 10.2 | 1.2 ± 0.5 | 2.6 ± 1.1 | 2.4 ± 0.6c | 4.2 ± 2.3 |
| CS25 | 27.1 ± 7.1 | 1.2 ± 0.4 | nd | 4.1 ± 2.1 | nd |
| CS40 | 33.5 ± 4.2 | 0.5 ± 0.3 | nd | 3.5 ± 0.7 | 1.3 ± 1.1 |