Table II. Label enrichments in 5-week-old mature root tips incubated in unilabeled [13C]Glc for antisense transgenic lines

Roots for mitochondrial mMDH, FL, SCoAL, ACO, and CS plants were incubated for 5 h. Two representative lines of each transgenic set were characterized. Values are presented as means ± se of six individual plants per line. Values marked with different letters differ significantly from each other (P < 0.05). Negligible enrichment is indicated with “nd.”

GenotypeCitrateFumarateIsocitrate (×10−4)Malate2-Oxoglutarate
μmol label accumulated expressed as hexose equivalents
Wild type31.7 ± 10.30.7 ± 0.12.7 ± 0.94.7 ± 0.92.7 ± 1.2
mMDH715.6 ± 4.70.3 ± 0.21.1 ± 0.3a3.3 ± 1.85.4 ± 1.9
mMDH869.0 ± 21.41.1 ± 0.62.9 ± 1.08.9 ± 1.6a17.6 ± 3.4a
mMDH217.3 ± 2.7a0.2 ± 0.1a3.3 ± 1.32.6 ± 0.81.0 ± 0.3
FL117.5 ± 2.0a0.3 ± 0.27.5 ± 2.1b0.9 ± 0.3b1.8 ± 1
FL415.2 ± 2.6a0.5 ± 0.14.4 ± 2.30.5 ± 0.2b2.8 ± 1.1
FL6326.2 ± 12.60.2 ± 0.1a18.1 ± 9.34 ± 1.318.4 ± 2.1a
SCoAL1838.8 ± 4.80.8 ± 0.3nd2.3 ± 0.3c7.7 ± 1.0b
SCoAL2533.7 ± 3.70.4 ± 0.1nd3.8 ± 1.13.5 ± 1.1
SCoAL4726.5 ± 5.70.7 ± 0.3nd5.5 ± 2.53.5 ± 2.0
ACO1920.6 ± 5.30.5 ± 0.1nd4.3 ± 1.02.4 ± 0.7
ACO2625 ± 8.30.8 ± 0.3nd6.6 ± 1.51.3 ± 0.2
ACO3814.6 ± 3.2b0.7 ± 0.4nd5.8 ± 2.02.4 ± 1.1
CS2232.9 ± 10.21.2 ± 0.52.6 ± 1.12.4 ± 0.6c4.2 ± 2.3
CS2527.1 ± 7.11.2 ± 0.4nd4.1 ± 2.1nd
CS4033.5 ± 4.20.5 ± 0.3nd3.5 ± 0.71.3 ± 1.1