Table I. Summary of cytochemical staining and immunolocalization of cell wall polymers in in vitro-grown Arabidopsis pollen tubes

Data are based on observations at the fluorescence microscopy (FM) and electron microscopy (EM) levels. −, No labeling detected (at over 500-ms exposure time); ±, weak labeling detected; +, strong labeling detected. iw, Inner wall layer; na, not applicable; nd, not determined; ow, outer wall layer.

TargetProbeBack from the TipTip
FM LevelEM LevelFM Level
β-GlucanCalcofluor white+na+
CalloseAniline blue+na
Anti-callosendiwnd
ExtensinLM1nd
AGPLM2±nd+
MAC207±nd+
JIM13nd
HGJIM5+nd±
JIM7±nd+
GalactanLM5±nd±
ArabinanLM6+ow+
LM13+nd+
XylogalacturonanLM8+nd+
XyGCCRC-M1+iw/ow+
LM15+iw/ow+
Controls