Table II. Monosaccharide composition and linkage analysis of total pollen tube cell wall and pectin-enriched (ammonium oxalate) and hemicellulose-enriched (KOH) extracts
Carbohydrate AnalysisPollen Tube WallOxalate ExtractKOH Extract
Neutral sugara (%)78.597.6
Uronic acidb (%)21.52.4
Monosaccharide compositionb
    Ara43.6 ± 4.524.7 ± 1.923 ± 7.4
    Fuc1.2 ± 0.21.0 ± 0.50.9 ± 0.7
    Gal8.4 ± 1.018.3 ± 0.614 ± 3.2
    Glc19.8 ± 5.119.3 ± 2.840.1 ± 6.6
    GalUA10.9 ± 0.721.5 ± 1.46.8 ± 0.2
    GlcUA2.2 ± 3.03.4 ± 0.81.5 ± 0.4
    Rha5.3 ± 0.84.2 ± 0.22.4 ± 0.7
    Man2.2 ± 1.51.7 ± 3.04.9 ± 0.1
    Xyl6.2 ± 1.25.8 ± 0.66.5 ± 0.4
Linkage analysisc
    t-Araf27.48.5
    5-Araf28.214.6
    2,5-Araf3.72.2
    3,5-Araf3.92.7
    t-Xyl1.41.1
    2-Xyl12.4
    t-Gal1.76
    3-Gal21.2
    4-Gal1.5nd
    6-Gal41.8
    3,6-Gal5.67.3
    4,6-Gal1.22.2
    3-Glc1027.6
    4-Glc8.415.4
    2,3-Glcnd7
  • a Determined by the phenol sulfuric and m-hydroxydiphenyl colorimetric assays.

  • b Determined by GC and expressed as mol %. Values are means ± sd from two independent experiments.

  • c Determined by GC-MS of partially methylated alditol acetates and expressed as percentage of total area of the identified peaks. t-Araf denotes 1,4-di-O-acetyl-1-deuterio-2,3,5-tri-O-methyl-d-arabinitol, etc. –, Not determined; nd, not detected.