Table V. Kinetic values for wild-type and mutant forms of SbCCR1, SbCCR2, Sobic.002G146000, and Sobic.010G066000 in the presence of feruloyl-CoA or p-coumaroyl-CoA

ND, Not determined.

PlantKcat (s−1)Kmm)Kcat/Kmm−1 s−1)
Feruloyl-CoAp-Coumaroyl-CoAFeruloyl-CoAp-Coumaroyl-CoAFeruloyl-CoAp-Coumaroyl-CoA
Wild type3.960.0570.21000.05660.0005
T154A0.740.02155700.00480.0003
Y310F0.47ND132ND0.0036ND
T154Y17.248.42631130.27360.0745
SbCCR23.240.50247140.01310.0358
Sobic.002G1460000.050.097.0520.00720.0017
Sobic.010G0660000.040.008.05.00.00490.0000