Table II. Thermodynamic properties of interaction between SbCCR1 and various ligands

ND, Not detected.

LigandKdΔHΔS
μmkcal mol−1cal mol−1 K−1
Feruloyl-CoA (apo form)13 ± 1.7−8.6 ± 0.6−6.4
Caffeoyl-CoA (apo form)7.2 ± 0.5−29 ± 1.9−72.8
p-Coumaroyl-CoA2.1 ± 0.2−28 ± 0.923.5
NADPH6.5 ± 0.6−6.1 ± 0.23.16
NADP+14 ± 4.7−3.1 ± 1.02.01
CoA25 ± 4.0−7.2 ± 1.0−3.14
NADHNDNDND
Ferulic acidNDNDND
Feruloyl-CoA (NADP+ bound)NDNDND
Caffeoyl-CoA (NADP+ bound)NDNDND