Table I. Comparisons of the expression levels of suberin and cutin biosynthetic and deposition-related genes in the wild type (Col-0) and myb107, showing the fold change of gene expression in myb107 versus the wild type quantified by RNA-seq and qRT-PCR

For repeat 1 and repeat 2 of the RNA-seq and qRT-PCR experiments, RNAs from mixed siliques of different growth stages were used. For repeat 3 of the qRT-PCR experiment, RNAs from stage III developing seeds were used. Fold change values less than 0.5 are highlighted in boldface. ND, Not determined.

Gene NameArabidopsis Genome Initiative No.PathwaysRNA-Seq (myb107/Wild Type)qRT-PCR (myb107/Wild Type)
Repeat 1Repeat 2Repeat 1Repeat 2Repeat 3
BAHD acyltransferase
 HHT/ASFTAT5G41040Suberin0.590.480.480.630.15
 FACTAT5G63560Suberin0.100.110.390.390.06
 DCFAT3G48720Cutin0.950.591.181.740.73
 DCRAT5G23940Cutin1.151.170.990.801.02
Cytochrome P450 monooxygenase
 CYP86A1AT5G58860Suberin0.490.170.490.570.59
 CYP86B1AT5G23190Suberin0.380.460.390.320.25
 CYP86A2AT4G00360Cutin0.890.870.760.760.63
 CYP86A8AT2G45970Cutin0.790.710.850.600.65
 CYP77A6AT3G10570Cutin1.160.921.220.601.23
Long-chain acyl-CoA synthetase
 LACS1AT2G47240Cutin1.000.92NDNDND
 LACS2AT1G49430Cutin/suberin0.570.620.400.450.49
Glycerol-3-phosphate acyltransferase
 GPAT5AT3G11430Suberin0.840.600.681.120.36
 GPAT4AT1G01610Cutin/suberin0.950.490.710.430.45
 GPAT6AT2G38110Cutin0.841.020.830.631.02
 GPAT8AT4G00400Cutin0.911.640.980.83ND
Alcohol-forming fatty acyl-CoA reductase
 FAR1AT5G22500Suberin0.330.530.200.370.08
 FAR4AT3G44540Suberin0.240.230.280.360.15
 FAR5AT3G44550Suberin0.570.590.360.670.28
3-Ketoacyl-CoA synthase
 KCS2AT1G04220Wax/suberin1.000.850.780.731.06
 KCS20AT5G43760Wax/suberin0.820.790.810.700.58
ABC transporter
 ABCG2AT2G37360Suberin0.530.72NDNDND
 ABCG6AT5G13580Suberin1.551.210.930.650.86
 ABCG20AT3G53510Suberin0.860.800.560.580.84
 ABCG11AT1G17840Cutin0.921.021.430.680.64