Table I. Summary of expression tags generated using two different sequencing methods
Data TypeSequencing Methods
454Illumina
TreatmentaN RN DT1T2T3N1N2N3
Totalb2.51 × 10+052.15 × 10+051.69 × 10+071.83 × 10+071.78 × 10+071.77 × 10+071.79 × 10+071.52 × 10+07
Mappedc2.01 × 10+051.68 × 10+051.09 × 10+071.24 × 10+071.15 × 10+071.07 × 10+071.13 × 10+079.86 × 10+06
Genicd1.08 × 10+051.32 × 10+058.96 × 10+069.43 × 10+061.02 × 10+078.33 × 10+068.82 × 10+067.81 × 10+06
Intergenice9.30 × 10+043.61 × 10+041.93 × 10+062.97 × 10+061.31 × 10+062.38 × 10+062.48 × 10+062.05 × 10+06
  • a Treatment types: N R, T1, T2, and T3 represent N-replete growth; N D, N1, N2, and N3 represent N-deprived growth. For Illumina, there are three biologically independent replicates for each treatment (T1–T3 and N1–N3).

  • b Number of sequencing reads after filtering out low-quality reads based on 454 and Illumina base-calling methods.

  • c Number of sequencing reads after mapping to the C. reinhardtii version 4.0 genome.

  • d Number of mapped sequencing reads overlapping with C. reinhardtii version 4.0-filtered gene models.

  • e Number of mapped sequencing reads not overlapping with any C. reinhardtii version 4.0-filtered gene models