Table I. FA composition of transgenic Arabidopsis seeds of T1 lines expressing the castor hydroxylase (CL37 background) and RcPDAT1A, RcPDAT1B, or RcPDAT2

The transgenic plant lines for each of these genes were obtained from independent transformation events. All data are averages of three independent measurements ± se. n.d., Not determined.

Transgenic LineFA CompositionSum of HFAs
16:018:018:118:218:320:118:1-OH18:2-OH
% of total
RcPDAT1A.111.5 ± 0.06.3 ± 0.133.6 ± 0.816.1 ± 0.17.3 ± 0.10.5 ± 0.019.4 ± 0.55.5 ± 0.124.9 ± 0.5
RcPDAT1A.211.5 ± 0.06.8 ± 0.333.3 ± 0.316.9 ± 0.57.9 ± 0.20.4 ± 0.217.6 ± 0.25.6 ± 0.323.2 ± 0.4
RcPDAT1B.111.0 ± 0.34.9 ± 0.243.6 ± 1.218.0 ± 0.37.3 ± 0.20.5 ± 0.011.4 ± 0.43.4 ± 0.214.8 ± 0.4
RcPDAT1B.212.1 ± 0.25.4 ± 0.140.9 ± 0.418.3 ± 0.47.2 ± 0.20.4 ± 0.012.5 ± 0.03.2 ± 0.115.7 ± 0.1
RcPDAT2.114.1 ± 1.95.7 ± 0.330.9 ± 4.724.4 ± 2.67.6 ± 0.5n.d.15.1 ± 0.22.1 ± 0.317.2 ± 0.3
RcPDAT2.217.5 ± 1.17.76 ± 0.320.8 ± 2.429.4 ± 1.98.8 ± 0.5n.d.15.3 ± 0.50.6 ± 0.315.8 ± 0.6
CL3711.2 ± 0.14.5 ± 0.042.6 ± 0.017.7 ± 0.06.6 ± 0.20.5 ± 0.013.5 ± 0.23.5 ± 0.117.0 ± 0.2
CL3714.4 ± 0.16.7 ± 0.332.5 ± 0.421.3 ± 0.27.3 ± 0.1n.d.14.5 ± 0.13.3 ± 0.017.8 ± 0.1