Table I. The COI1-dependent JA-regulated proteins

A total of 35 COI1-dependent JA-regulated proteins were classified based on the biological process in which the gene product participated, and their corresponding spots are shown on Figure 1. Col-0, Ecotype Columbia.

FunctionSpot No.Gene LocusProtein NameJA-Treated coi1-1/JA-Treated Col-0JA-Treated coi1-1/Water-Treated coi1-1Water-Treated coi1-1/Water-Treated Col-0Mowse ScoreNo. of Matching PeptidesbProtein Coveragec
RatioaPRatioaPRatioaP
%
Storage protein1At5g24780deVegetative storage protein 1−30.711.80E-08−1.765.60E-01−2.052.10E-013.43E+061338
2At5g24770dVegetative storage protein 2−44.801.80E-07−1.756.20E-01−2.712.10E-011.31E+061040
JA biosynthesis protein3At3g45140dfgLipoxygenase 2−8.442.20E-05−1.077.90E-01−.305.70E-015.38E+091925
4At3g45140dfgLipoxygenase 2−6.369.00E-06−1.047.10E-01−.157.40E-011.21E+122137
5At3g45140dfgLipoxygenase 2−6.285.40E-07−1.047.10E-01−1.175.10E-021.63E+132236
6At3g25770deAllene oxide cyclase 2−4.552.00E-05−1.146.50E-01−1.093.70E-016.83E+03632
Jacalin lectin family protein7At3g16420dJacalin-related lectin 30−2.263.50E-05−1.512.30E-01−1.252.30E-013.38E+061152
8At3g16450dJacalin lectin family protein−1.921.90E-03−1.195.20E-01−1.181.60E-011.66E+05736
9At3g16460dJacalin lectin family protein−1.862.10E-021.164.80E-01−1.091.58E+002.28E+04613
Amino acid synthesis and modification protein10At4g23600dCystine lyase−6.981.50E-051.273.40E-011.235.70E-028.63E+051025
11At4g14880Cytosolic O-acetylserine(thiol)lyase−2.388.40E-05−1.048.90E-011.113.50E-012.78E+05830
12At3g54640dTrp synthase α-subunit−1.505.30E-031.065.10E-011.056.40E-015.10E+05736
13At5g17920Met synthase−0.647.60E-03−1.047.40E-013.046.30E-021.08E+131933
14At4g33010Gly decarboxylase P-protein 11.994.90E-041.955.40E-012.441.40E-017.79E+091725
15At4g33010Gly decarboxylase P-protein 11.706.10E-041.126.70E-011.941.70E-014.55E+081421
Cellular respiration protein16At2g44350fCitrate synthase1.162.00E-021.215.70E-011.212.80E-015.52E+111748
17At1g42970dfGlyceraldehyde-3-phosphate dehydrogenase B subunit1.531.50E-041.332.80E-011.302.50E-017.70E+061431
18At5g11670Putative malate oxidoreductase1.381.00E-021.067.00E-011.471.20E-014.64E+101735
Defense-related protein19At1g19570dfgDehydroascorbate reductase−2.891.20E-06−1.173.60E-01−1.181.70E-011.01E+07859
20At3g12490Protein with Cys proteinase inhibitor activity−1.667.50E-04−1.403.50E-01−1.581.50E-016.33E+04639
21At1g52400dβ-Glucosidase homolog 1−4.948.50E-06−1.249.70E-01−1.271.04E+004.88E+071237
22At1g52400dβ-Glucosidase homolog 1−2.441.40E-04−1.127.80E-01−1.271.20E-011.88E+05824
23At5g169702-Alkenal reductase1.723.20E-051.648.40E-021.059.40E-014.01E+05833
Translation elongation factor protein24At1g56070Translation elongation factor 2-like protein1.681.60E-031.446.00E-011.041.80E-011.21E+091622
25At1g56070Translation elongation factor 2-like protein2.011.10E-031.147.10E-012.991.10E-015.71E+102226
Photosynthesis- and chlorophyll-related protein26At3g27890NAD(P)H:quinone reductase−1.733.30E-05−1.302.10E-01−1.029.50E-011.76E+04633
27At3g04790Ribose 5-phosphate isomerase-related−1.342.00E-03−1.305.20E-01−1.039.00E-011.57E+06950
28AtCG00480β-Subunit of ATP synthase−2.627.40E-051.401.60E-011.325.10E-011.24E+091336
29At3g60750fPutative transketolase1.463.30E-031.264.90E-012.081.00E-012.04E+101627
30At1g32060Phosphoribulokinase1.812.40E-03−1.119.40E-011.251.20E-012.23E+091246
31AtCG00490fRubisco large chain1.589.10E-041.304.90E-011.146.20E-014.20E+081632
32AtCG00490fRubisco large chain1.683.50E-021.592.00E-011.145.50E-011.59E+051024
33At2g39730fRubisco activase1.503.40E-041.242.60E-01−1.039.20E-011.80E+081131
34At3g55800gSedoheptulose-1,7-bisphosphatase1.573.60E-04−1.143.80E-011.201.80E-012.53E+071336
Other protein35At5g24420dGlucosamine/galactosamine-6-phosphate isomerase-related−2.352.60E-021.156.00E-01−1.278.70E-011.43E+04527
36At5g25980Myrosinase (thioglucoside glucohydrolase)−1.873.00E-031.156.60E-01−1.009.70E-011.20E+101633
37At3g16470dgJA-inducible protein isolog−3.432.90E-05−1.039.30E-01−1.118.10E-017.32E+05834
38At3g16470dgJA-inducible protein isolog−5.651.70E-07−1.077.20E-01−1.212.20E-011.95E+081040
39At3g16470dgJA-inducible protein isolog−2.515.20E-051.163.90E-01−1.066.50E-013.05E+04728
40At3g14540Terpene synthase/cyclase family protein−2.343.60E-08−1.393.30E-01−1.189.00E-019.64E+03514
41At5g65020dAnnexin Arabidopsis 2−1.642.40E-04−1.027.50E-011.557.50E-015.13E+04826
42At4g01870tolB protein-related2.227.20E-043.401.10E-011.959.50E-021.03E+091232
43At3g13470GroEL-like chaperone1.315.00E-031.019.30E-011.488.20E-021.37E+081330
  • a Average volume ratio quantified by Decyder 6.5 biological variation analysis module.

  • b Number of peptide masses matching the top hit from MS-Fit peptide mass fingerprinting.

  • c Amino acid sequence coverage for the proteins.

  • d JA-regulated genes identified previously in Arabidopsis by microarray analysis.

  • e JA-regulated proteins identified previously in Arabidopsis.

  • f Similar JA-regulated proteins identified in rice.

  • g OPDA-regulated proteins identified previously in Arabidopsis.