Table II. Overview and representation in the microarray of genes known to be regulated by heat shock in Chlamydomonas with FC ≥ 10

ER, Endoplasmic reticulum.

IdentifierGeneFCComment
32.AHSP22A91,297Chloroplast heat shock 22-kD protein A (Schroda, 2004)
9516.EHSP22B45,023Chloroplast heat shock 22-kD protein B (Schroda, 2004)
9984.EHSP22C6,570Chloroplast heat shock 22-kD protein C (Schroda, 2004)
4176.CDNJ33,050DnaJ-like protein, fusion protein containing a DnaJ domain (N terminal) and a PDI domain (C terminal); similar to human ERdj5 protein, an ER resident protein
9769.ECLPB12,909Cytosolic ClpB chaperone, Hsp100 family
9317.EHSP22F600Chloroplast heat shock 22-kD protein F (Schroda, 2004)
8031.DHSP90C315Heat Shock Protein 90C, isoform 3, targeted to chloroplast stroma (Willmund and Schroda, 2005)
7358.CHSP90B240HSP90B is one of three HSP90 genes in Chlamydomonas; HSP90B is localized to the ER (Schroda, 2004; Willmund and Schroda, 2005)
9825.EDNJ21213DnaJ-like protein; contains N-terminal J-domain and DnaJ C-terminal region
9479.EHSP70E168HSP70E is one of nine genes encoding HSP70-like proteins in Chlamydomonas; it is more similar to HSP110-like proteins, as is HSP70G; HSP70E is inducible by MgProto and light (Schroda, 2004; Vasileuskaya et al., 2004)
9728.EBIP1/BIP296BIP2 is one of nine genes encoding HSP70-like proteins in Chlamydomonas; BIP2 contains an ER retention motif, an ER-targeting sequence, and is most similar to ER-HSP70s from other organisms; it forms a cluster with ER-targeted chaperones HSP90B and BIP1 (Schroda, 2004)
291.AHSP70A93HSP70A is one of nine genes encoding HSP70-like proteins in Chlamydomonas; the gene is inducible by light, MgProto, and heme and encodes a cytosolic protein
342.ACPN60A82GroEL/HSP60 homolog, targeted to the chloroplast; called Cpn60-α in higher plants; inducible by heat shock (Thompson et al., 1995; Schroda, 2004)
5371.CCLPB376ClpB chaperone, Hsp100 family; probably targeted to chloroplast or mitochondria
893.CCPN23/ MSCL26720-kD chaperonin, chloroplast precursor (protein Cpn21)
8824.DHSP90A58HSP90A is one of three HSP90 genes in Chlamydomonas; HSP90A is localized to the cytosol (Schroda, 2004; Willmund and Schroda, 2005)
1539.CCDJ155Chloroplast DnaJ-like protein
6484.CHSP70G47HSP70G is one of nine genes encoding HSP70-like proteins in Chlamydomonas; it is more similar to HSP110-like proteins, as is HSP70E; probably located in the ER lumen
4875.CHSP70G41Array probe 2 for the same HSP70G as probe 6484.C
8957.EHSP70C38HSP70C is one of nine genes encoding HSP70-like proteins in Chlamydomonas; HSP70C is localized to mitochondria; inducibility by heat shock or light was demonstrated previously (von Gromoff et al., 1989; Schroda, 2004)
4157.CP2324P23 cochaperone of HSP90 system (N terminus); probably cytosolic (by homology)
479.ACPN60B124Chaperonin 60B1, GroEL/HSP60 homolog, targeted to the chloroplast; inducibility by heat shock was demonstrated previously (Thompson et al., 1995; Schroda, 2004)
8349.DDNJ121DnaJ-like protein; probably cytosolic
7409.CCPN202120-kD chaperonin, chloroplast precursor (protein Cpn21)
5763.CERJ120DnaJ-like protein; probably targeted to ER
229.ACPN60B219GroEL/HSP60 homolog, targeted to the chloroplast; inducibility by heat shock was demonstrated previously (Thompson et al., 1995)
464.AHSP70B14One of nine genes encoding HSP70-like proteins in Chlamydomonas; it is more similar to HSP110-like proteins, as is HSP70E; HSP70B is located in the chloroplast
118.ACGE113Chloroplast GrpE homolog, nucleotide release factor, cochaperone of HSP70B (Liu et al., 2005)