Table II. Summary of Lys modification sites in eEF-1A
PositionModified PeptidesModificationzm/zd m/zP ValueComment
K36LGGID(ac)KRAcetyl_K2400.7290.0062.46E − 06
K36LGGID(trimethyl)KRTrimethyl_K2400.7290.0242.46E − 06
K44FE(dimethyl)KEAAE(ox)MNKRDimethyl_K3466.2060.0280.00E + 00
K44FE(methyl)KEAAEMNKRMethyl_K3456.2040.0270.00E + 00
K55SF(dimethyl)KYAWVLDKDimethyl_K2642.7730.0802.51E − 10
K79GITIDIALW(ac)KFETTKAcetyl_K2889.4320.0510.00E + 00
K79GITIDIALW(trimethyl)KFETTKTrimethyl_K2889.4320.0690.00E + 00
K187VGYNPD(ac)KIPFVPISGFEGDNMIERAcetyl_K3912.6460.1371.39E − 33
K187VGYNPD(trimethyl)KIPFVPISGFEGDNMIERTrimethyl_K3912.6460.1491.39E − 33
K227GPTLLEALDQINEP(ac)KRPSDKPLRAcetyl_K3877.0210.1272.18E − 17Confirmed by Ab
K227GPTLLEALDQINEP(trimethyl)KRPSDKPLRTrimethyl_K3877.0210.1392.18E − 17
K306NVAV(ac)KDLKAcetyl_K2465.249−0.4734.02E − 10Confirmed by Ab