Table I. Plastid-localized Lys-acetylated proteins

Immunoenriched Lys-acetylated peptides from Arabidopsis leaves and chloroplasts were analyzed with the LTQ Velos/LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific; Supplemental Table S2). Spectra files were processed using the CPFP as described in “Materials and Methods.” Fragment ion spectra were manually inspected for indicative b- and y-ion series (Supplemental Fig. S1). i-probability shows confidence score of Peptide Prophet (CPFP; Trudgian et al., 2010).

DescriptionProteinPeptideLys (K) Side Chain Sitei-probability
Fru-bisP aldolaseAt2g21330K.YTGEGESEEAKEGMFVK(ac)GYTY.-K3951
GAPA-2 (GAPDH)At1g12900K.LNGIALRVPTPNVSVVDLVVQVSK(ac)K.TK3140.95
PGK1At3g12780K.GVTPK(ac)FSLAPLVPR.LK1461
PGK1At3g12780K.IGVIESLLEK(ac)CDILLLGGGMIFTFYK.AK2881
PGK1At3g12780R.LSELLGIEVTK(ac)ADDCIGPEVESLVASLPEGGVLLLENVR.FK1691
RCA (Rubisco activase)At2g39730K.NFLTLPNIKVPLILGIWGGK(ac)GQGK.SK1671
Large subunit of RubiscoAtCg00490K.ASVGFK(ac)AGVK.EK140.98
Large subunit of RubiscoAtCg00491K.AGVK(ac)EYK.LK180.99
Large subunit of RubiscoAtCg00490R.IPPAYTK(ac)TFQGPPHGIQVER.DK1461
Large subunit of RubiscoAtCg00490K.YGRPLLGCTIK(ac)PK.LK1751
Large subunit of RubiscoAtCg00490K.AGVKEYK(ac)LTYYTPEYETK.DK210.98
Large subunit of RubiscoAtCg00490K.GHYLNATAGTCEEMIK(ac)R.AK2521
Large subunit of RubiscoAtCg00490R.VLAK(ac)ALR.LK3160.95
Large subunit of RubiscoAtCg00490R.DDYVEK(ac)DR.SK3560.99
Large subunit of RubiscoAtCg00490K.WSPELAAACEVWK(ac)EITFNFPTIDKLDGQE.-K4630.85
Rubisco small subunit 1A (RBCS-1A)At1g67090R.YWTMWK(ac)LPLFGCTDSAQVLK.EK1260.96
ATP synthase F1 sector subunit βAtCg00480K.LSIFETGIK(ac)VVDLLAPYRR.GK1541
PSI reaction center, putative/PSI-HAt1g52230K.FFETFAAPFTK(ac)R.GK991
CAB3 (chlorophyll a/b-binding protein 3)At1g29910R.NGVK(ac)FGEAVWFK.AK1250.74
GSH2At5g27380K.MESQK(ac)PIFDLEKLDDEFVQK.LK661
AAC1 (ADP/ATP carrier 1)At3g08580R.MMMTSGEAVK(ac)YK.SK3251
GS2 (Gln synthetase 2)At5g35630K.WPLGWPVGAFPGPQGPYYCGVGADK(ac)IWGR.DK2231
Catalytic/coenzyme-binding chloroplast precursorAt2g37660R.TGQIVYK(ac)K.LK940.62