Table III. Seed germination tests in the presence and absence of spectinomycin
LineaMedium with SpectinomycinMedium without Spectinomycin
Seeds PlatedSeeds GerminatedMosaic CotyledonsSeeds PlatedSeeds GerminatedMosaic Cotyledons
Total%Total%Total%
211,0952,14019.3492.31,7831,21968.40
310,4451,3571315811.61,79065536.60
67,6564,24155.4280.61,8231,36674.90
Wt1,6481,23775.10b0b1,3691,02574.90
  • a Lines 2, 3, and 6 refer to CAB lines grown from T1 seed obtained from parental lines 2, 3, and 6 in the R2 regeneration cycle (Fig. 3). Line Wt refers to the wild-type control line.

  • b Cotyledons were completely white/bleached, indicating no spectinomycin resistance.