Table I. Ratios of target gene transcript to actin in control, IAA-, and ACC-treated wild-type samples as determined by qRT-PCR

Roots were transferred to control medium or medium containing 1 μm ACC or IAA for 4 h. RNA was harvested from roots and amplified by qRT-PCR with gene-specific primers. The average ± se of three biological replicates is reported. Fold change, shown in parentheses, was determined by dividing the target actin ratios of the treated sample by the control ratio. ND, Not detected.

GeneTranscript Abundance Relative to Actin
ControlIAAACC
CHS0.085 ± 0.0080.28 ± 0.06 (3.3)0.25 ± 0.05 (3.0)
CHI0.048 ± 0.0060.19 ± 0.03 (3.9)0.16 ± 0.02 (3.3)
F3H0.011 ± 0.0020.011 ± 0.003 (1.0)0.012 ± 0.002 (1.1)
FLS0.023 ± 0.0040.10 ± 0.02 (4.5)0.065 ± 0.004 (2.8)
F3′H0.0059 ± 0.00060.022 ± 0.003 (3.7)0.0089 ± 0.0005 (1.5)
DFRNDNDND
MYB120.022 ± 0.0020.31 ± 0.05 (14.2)0.15 ± 0.04 (6.9)
TTG10.0082 ± 0.00040.033 ± 0.003 (4.1)0.027 ± 0.004 (3.3)
PAP10.015 ± 0.0040.058 ± 0.004 (3.9)0.047 ± 0.006 (3.1)