Table II. DPBA fluorescence is altered in tir1-1, ein2-5, and myb12
GenotypeTreatmentQuercetinKaempferolQ/K Ratio
Relative DPBA Fluorescencea
ColumbiaUntreated1.00 ± 0.060.40 ± 0.022.50 ± 0.23
ColumbiaIAA3.14 ± 0.05b,c1.04 ± 0.02b,c3.03 ± 0.20
ColumbiaACC1.63 ± 0.04b,c1.32 ± 0.03b,c1.24 ± 0.08b,c
ColumbiaIAA and ACC4.30 ± 0.13b,c2.02 ± 0.07b,c2.13 ± 0.35
tir1-1Untreated0.39 ± 0.02b0.24 ± 0.02b1.60 ± 0.10b
tir1-1IAA0.50 ± 0.05b0.27 ± 0.04b1.87 ± 0.26b
tir1-1ACC1.01 ± 0.09b,c0.77 ± 0.06b,c1.32 ± 0.08b,c
ein2-5Untreated0.60 ± 0.04b0.20 ± 0.02b3.00 ± 0.34
ein2-5IAA2.53 ± 0.18b,c0.62 ± 0.06b,c4.11 ± 0.45b,c
ein2-5ACC0.54 ± 0.08b0.27 ± 0.02b,c2.01 ± 0.21
myb12Untreated0.62 ± 0.03b0.28 ± 0.02b2.18 ± 0.18
myb12IAA0.74 ± 0.03b0.29 ± 0.02b2.53 ± 0.20
myb12ACC0.64 ± 0.03b0.30 ± 0.02b2.16 ± 0.23
  • a Normalized DPBA fluorescence relative to Q-DPBA in untreated control is shown for samples treated for 8 h with 1 μm IAA or ACC. Mean ± se for greater than 15 individuals collected with identical settings is shown, as is the average Q/K ratio.

  • b Values that are significantly different from the untreated wild type for each category (P < 0.05).

  • c Values that are significantly different from the untreated control within a genotype within each category (P < 0.05).